Ukubeka iliso kwi-intanethi kumxholo we-glucose ukwenzela ukutya kwexesha langempela ukuqinisekisa ukugqitywa kakuhle kwenkqubo yokuvutshelwa.
Ubunjineli be-Biofermentation lelinye lamacandelo abalulekileyo kubunjineli banamhlanje be-biopharmaceutical, ukufumana iimveliso ezifunwayo ze-biochemical ngokusebenzisa inkqubo yokukhula kwee-microorganisms.Inkqubo yokukhula kwe-microbial ibandakanya izigaba ezine: isigaba sokulungelelanisa, isigaba sokungena, isigaba sokumisa, kunye nesigaba sokufa.Ngexesha lesigaba sokumisa, isixa esikhulu seemveliso ze-metabolic ziqokelelana.Eli ikwalixesha apho iimveliso zivunwa kwiimpendulo ezininzi.Emva kokuba esi sigaba sigqithisiwe kwaye isigaba sokufa singenisiwe, zombini umsebenzi weeseli ze-microbial kunye nokucoceka kweemveliso kuya kuchaphazeleka kakhulu.Ngenxa yokuntsonkotha kokuchaphazeleka kwebhayoloji, ukuphinda-phinda kwenkqubo yokuvubela kubi, kwaye ulawulo lomgangatho kulucelomngeni.Njengoko inkqubo inyuka ukusuka kwilabhoratri ukuya kwisikali sokulinga, kwaye ukusuka kwisikali sokulinga ukuya kwimveliso enkulu, ukungaqhelekanga kwiimpendulo kunokwenzeka ngokulula.Ukuqinisekisa ukuba intshukumo yokubilisa igcinwa kwisigaba sokumisa kangangexesha elongeziweyo ngowona mba uxhalabisayo xa kunyuswa ubunjineli bokubila.
Ukuqinisekisa ukuba i-microbial strain ihlala kwinqanaba elikhulayo kwaye lizinzile ngexesha lokuvutshelwa, kubalulekile ukugcina umxholo we-metabolites yamandla efunekayo njengeglucose.Ukusebenzisa i-spectroscopy ye-intanethi ukujonga umxholo we-glucose kwi-broth fermentation ngexesha langempela yindlela efanelekileyo yobugcisa yokulawula inkqubo ye-biofermentation: ukuthatha utshintsho kwi-concentration ye-glucose njengemilinganiselo yokuncedisa kunye nokumisela imeko ye-microbial strain.Xa umxholo uwela ngaphantsi komda omiselweyo, ukuxhaswa kunokwenziwa ngokukhawuleza ngokusekelwe kwiziphumo zokubeka iliso, ukuphucula kakhulu umgangatho kunye nokusebenza kakuhle kwe-biofermentation.Njengoko kubonisiwe kulo mzobo ungezantsi, isebe elisecaleni lizotywa kwitanki encinci yokuvubela.I-spectroscopy probe ifumana imiqondiso yolwelo yexesha lokwenyani ngedama lokujikeleza, ekugqibeleni ivumela ukuchongwa koxinzelelo lweglucose kulwelo lokubilisa ukuya kuthi ga kwi-3 ‰.
Kwelinye icala, ukuba iisampulu engaxhunyiwe kwi-intanethi yomhluzi wokubilisa kunye novavanyo lwelabhoratri isetyenziselwa ulawulo lwenkqubo, iziphumo zokubhaqwa okulibazisekayo zinokuphosa elona xesha lifanelekileyo lokongeza.Ngapha koko, inkqubo yesampulu inokuchaphazela inkqubo yokuvubela, njengokungcoliswa ziibhaktheriya zangaphandle.
Ixesha lokuposa: Dec-07-2023