Ukubeka iliso kwi-Intanethi kuqinisekisa ukuba umxholo we-substrate ungaphantsi komqobo, uqinisekisa umsebenzi we-enzyme yebhayoloji kuyo yonke le nkqubo, kunye nokunyusa izinga lokuphendula kwe-hydrolysis.
Iikhompawundi ze-Amide zibalulekile kwi-organic synthesis intermediates kunye neekhemikhali kwaye zisetyenziswa kakhulu kumayeza, amayeza okubulala izitshabalalisi, ukutya, ukukhuselwa kwendalo, ukuveliswa kweoli kunye nezinye iindawo.I-hydrolysis reaction yeqela le-nitrile kwiqela le-amide yenye yeendlela ezibalulekileyo zokulungiselela i-amide compounds kwishishini.
I-biocatalyst isetyenziswe kwinkqubo ye-green synthesis ye-amide compound ethile, kwaye umsebenzi wayo uchaphazeleka kakhulu kugxininiso lwe-substrate kunye nemveliso kwinkqubo.Ukuba i-concentration ye-substrate iphezulu kakhulu, i-catalyst iya kucinywa ngokulula, okwenza kube nzima ukuqhubeka nokusabela kwe-synthesis;ukuba ugxininiso lwemveliso luphezulu kakhulu, luya kukhokelela ekuqokeleleni kwe-substrate kunye nokusebenza kakuhle kwe-synthesis.Ukuze kuqinisekiswe owona msebenzi ulungileyo we-biological enzyme catalysts kwi-synthesis reaction, iindlela ezisebenzayo zobugcisa ziyafuneka ukubeka iliso kunye nempendulo ukulungelelanisa ukugxila kwe-nitrile substrates kunye neemveliso ze-amide ngexesha lokwenyani ngexesha lenkqubo yokusabela.
Okwangoku, iindlela ezinje ngesampulu ngamaxesha amiselweyo kunye nokwenza i-gas chromatography-mass spectrometry emva kwesampulu yonyango lwangaphambili zihlala zisetyenziselwa ukufumanisa i-substrate kunye nomxholo wemveliso kwinkqubo yokusabela.Ukufunyanwa kweziphumo ezingaxhunyiwe kwi-intanethi, imeko yangoku yokusabela ayinakwaziwa ngexesha lokwenyani, kwaye kunzima ukwenza ulawulo lwengxelo kunye nohlengahlengiso lomxholo we-substrate, kwaye elona thuba lokutyisa linokuphoswa.Itekhnoloji yokuhlalutya okubonwayo kwi-Intanethi ineengenelo zesantya sokubona ngokukhawuleza kwaye akukho mfuneko yonyango lwangaphambili.Inokuqonda ngokukhawuleza, ixesha lokwenyani, in-situ kunye nohlalutyo oluhlakaniphile lwenkqubo yokusabela, kwaye ineenzuzo ezikhethekileyo kwi-green synthesis ye-amide compounds.
Lo mfanekiso ungasentla ubonisa uhlolo lwe-intanethi lwenkqubo yokulungisa i-acrylamide ngokusabela kwe-bioenzymatic yekhompawundi ethile ye-nitrile.Ukusuka ku-0 ukuya ku-t1 emva kokuba kuqale ukusabela, isantya sokutyisa se-nitrile ekrwada sikhulu kakhulu, kwaye isantya sokuqokelelwa kwazo zombini i-substrate kunye nemveliso ikhawuleza kakhulu.Kwi-t1, umxholo we-substrate usondele kumda ophezulu womda.Ngeli xesha, abasebenzi bemveliso banciphisa izinga lokutya kwezinto ezikrwada ukugcina ugxininiso lwe-substrate kwinkqubo yokusabela ngaphakathi koluhlu olulawulekayo, kwaye imveliso isenokuqokelela ngokukhawuleza.Ekugqibeleni, xa impendulo iqhubela kwixesha le-t2, umxholo wemveliso uqokelela kwinqanaba ekujoliswe kulo, kwaye abasebenzi bemveliso bayayeka ukongeza izinto eziluhlaza ze-nitrile.Emva koko, inqanaba le-substrate lisondela kwi-zero kwaye umxholo wemveliso nawo udla ngokuzinza.Ngexesha lenkqubo yonke eqhubekayo yokuvelisa, ukubeka iliso kwi-intanethi kuqinisekisa ukuba i-enzyme yebhayoloji ye-catalytic reaction iqhubeka ngokufanelekileyo.
Kwi-synthesis enkulu, itekhnoloji yokubeka iliso kwi-Intanethi ibaluleke kakhulu.Ulwazi lwexesha lokwenyani lwe-substrate kunye nogxininiso lwemveliso lunokunceda impendulo ukulungisa umxholo we-substrate ngaphakathi koluhlu olufanelekileyo.Ngexesha lenkqubo yokusabela, inokwandisa umsebenzi we-catalyst ye-enzyme yebhayoloji, iphucule ukusebenza kakuhle kokusabela kwe-synthesis, kwaye incede ukulawula iiparamitha zenkqubo kwimeko efanelekileyo.Yandisa ubomi benkonzo ye-biological enzyme catalysts kwaye wandise izibonelelo.
Ixesha lokuposa: Jan-23-2024